Table 2. Nucleotide and amino acid variations of E2 gene in the study samples in comparison with ECSA lineage and other Indian samples are summarized below.

Nucleotide position	Amino acid variation	Samples
		ECSA	Indian samples	All study samples	KC977314	KJ57760
9437*	S299N	G	A/G	A
9476*	T312M	C	T/C	T
9556*	W339R	T	T/C	.	A
9571*	A344T	G	A	A
9587*	H349P	A	.	.		C
9664*	S375T	T	A/T	A
9698*	V386A	T	C/T	G