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. 2018 May 15;8:7546. doi: 10.1038/s41598-018-25774-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intercalated disc organization is disrupted with the loss of CENP-F in cardiomyocytes. Cardiomyocytes isolated from wild-type (a) and CENP-F^−/− (a’) mice were fixed and immunostained with alpha-actinin 2 antibody (teal) and TOPRO (orange). In CENP-F^+/+ cardiomyocytes, there are multiple junction ends, as indicated by * (a). However, in CENP-F^−/− cardiomyocytes, the cell termini is blunted, indicated by * (a’). The intercalated discs were immunostained with beta-catenin antibody (white) and the images are zoomed in to depict the lining of the cardiomyocyte junctions. In wild-type mice, there is thin and distinct junction staining (b) opposed to disintegrated and thickened junction staining of the mutant cardiomyocytes (b’). An EM image of wild-type heart shows a clear depiction of an intercalated disc, see horizontal structure pointed to by line (c), In CENP-F^−/− cardiomyocytes, the intercalated disc is highly disintegrated (c’). A gene expression analysis of beta-catenin in wild-type versus CENP-F^−/− cardiomyocytes (d). A western blot analysis of beta-catenin in wild-type versus CENP-F^−/− cardiomyocytes showed significant decrease in beta-catenin. 2 cropped samples are presented and n = 5, p < 0.05. (Full-length gels are presented in Supplementary Fig. 4). Scale bars: (a) 20 mm; (a’) 20 mm; (c) 2 microns; (c’) 2 microns. Error bars in (e) represent standard error of the mean (SEM).