6.
The growth curve of MC38 xenografts in mice following chemotherapy combined with immunotherapy. When the volume of the tumor reached 500 mm3, the chemotherapeutic drugs 5-Fu/oxaliplatin and/or phosphate buffered saline (PBS) were intraperitoneally injected in mice. The immune therapies were conducted twice on d 3 and d 7 following injection of the chemotherapeutic drugs [intravenous injection of Toll-like receptor 9 (TLR9) ligand and CD1d-MC38/α-galactosylceramide (α-GC) compound, respectively]. A total of 6 mice were used in each group. The inoculated tumors were observed every 2 or 3 d. The maximum and minimum diameters of the tumors were measured in order to calculate the tumor volume. (A) Chemotherapy for MC38 xenografts in mice; (B) Chemotherapy combined with immunotherapy for MC38 xenografts in mice. *, P<0.05; **, P<0.01; ***, P<0.001; ns, no significance.