1.

Grouping and time points of chemotherapy and immunotherapy

Group	D0	D3	D7
PBS, phosphate buffered saline; 5-Fu, 5-fluorouracil; OX, oxaliplatin; TLR, Toll-like receptor; α-GC, α-galactosylceramide.
Blank control group	PBS	PBS	PBS
5-Fu alone group	5-Fu	PBS	PBS
OX alone group	OX	PBS	PBS
Combined chemotherapy group	5-Fu + OX	PBS	PBS
Immunotherapy alone group	PBS	TLR9+CD1d-MC38/α-GC	TLR9+CD1d-MC38/α-GC
5-Fu + immunotherapy group	5-Fu	TLR9+CD1d-MC38/α-GC	TLR9+CD1d-MC38/α-GC
OX + immunotherapy group	OX	TLR9+CD1d-MC38/α-GC	TLR9+CD1d-MC38/α-GC
Combined chemotherapy + immunotherapy group	5-Fu + OX	TLR9+CD1d-MC38/α-GC	TLR9+CD1d-MC38/α-GC