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. 2018 May 15;8:7569. doi: 10.1038/s41598-018-25853-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The sialic acid specificity of WGA induces endothelial TNT formation and F-actin rearrangements. (A) Examples of cell masked stained HUVECs following 30 mins incubation with the stated lectins (10 μg/ml). Scale bar is 50 μm. (B) Mean data and analysis of the effect of lectins on the percentage of TNTs to cells (n = 3, control N = 28, WGA N = 29, Succin WGA N = 30, UEA1 N = 30, ConA N = 29, peanut N = 30). Data are represented as mean ± SD, *P < 0.05. (C) Phalloidin staining (red) indicates F-actin redistribution in HUVECs treated with 10 μg/ml WGA for 30 mins as compared to control cells or cells treated with 10 μg/ml succinylated WGA. Scale bar is 50 μm.