Fig. 3.

Expression and purification of C. jejuni-specific scFv antibodies. a Western blot of scFv61 and scFv80, expressed in E. coli and captured by affinity beads. Both antibodies have 6xHis tag at their C-terminus and were detected using His-probe. Nickel beads were used to capture the soluble antibodies. b Western blot of scFv80 expressed and purified using different imidazole concentrations in the binding buffer. c Western blot of scFv80 expressed and purified using different imidazole concentrations in the washing buffer. d Western blot of scFv80 expressed and purified using different imidazole concentrations in the elution buffer