Fig. 4.

Purified scFv80 antibody specifically recognizes C. jejuni cells. a In the ELISA assay, purified scFv80 was used to detect C. jejuni and other foodborne pathogens. The same amount of bacterial cells and scFv80 antibody was used in all the ELISA wells. Bound scFv80 was detected by HisProbe-HRP Conjugate and the substrate TMB (E). Signal read at 450 nm was shown in the Y-axis. Each column represents the mean and standard deviation of signals in three wells. b Recovery of C. jejuni by IMS from solutions containing different cell concentrations (10³–10⁷ cfu/ml) using scFv80 coated beads. Cell concentrations are labeled below each column. Recovery was calculated according to qPCR quantification data of the captured and input cells. c Recovery of Campylobacter cells by IMS using scFv80 coated beads from a mixed culture of C. jejuni, C. coli, and C. lari (each present at 10⁴ cfu/ml). qPCR reactions specific to C. jejuni, C. coli, or C. lari were used to quantify the input and captured cells before calculating the recovery rate of each species