Table 1.

Details of PCR primers employed for construction of rabbit scFv fragments. Short linker sequences (GGSSRSS), underlined, are based on Protocol 9.7, Barbas et al. (2001)

Primer type	Primer name	Primer ID	Primer sequence
Variable Kappa light chain	Vκ5′ Forward	RSCVK1	5′ GGG CCC AGG CGG CCG AGC TCG TGM TGA CCC AGA CTC CA 3′
		RSCVK2	5′GGG CCC AGG CGG CCG AGC TCG ATM TGA CCC AGA CTC CA 3′
		RSCVK3	5′ GGG CCC AGG CGG CCG AGC TCG TGA TGA CCC AGA CTG AA 3′
	Vκ 3′ Reverse	RKB9J1o-B	5′ GGA AGA TCT AGA GGA ACC ACC TTT GAT TTC CAC ATT GGT GCC 3′
		RKB9Jo-B	5′ GGA AGA TCT AGA GGA ACC ACC TAG GAT CTC CAG CTC GGT CCC 3′
		RKB42Jo-B	5′ GGA AGA TCT AGA GGA ACC ACC TTT GAC SAC CAC CTC GGT CCC 3′
Variable Lambda light chain	Vλ 5′ Forward	RSCλ1	5′ GGG CCC AGG CGG CCG AGC TCG TGC TGA CTC AGT CGC CCT C 3′
	Vλ 3′ Reverse	RJλo-B	5′ GGA AGA TCT AGA GGA ACC ACC GCC TGT GAC GGT CAG CTG GGT CCC 3′
Variable heavy chain	VH 5′ Forward	RSCVH1	5′ GGT GGT TCC TCT AGA TCT TCC CAG TCG GTG GAG GAG TCC RGG 3′
		RSCVH2	5′ GGT GGT TCC TCT AGA TCT TCC CAG TCG GTG AAG GAG TCC GAG 3′
		RSCVH3	5′ GGT GGT TCC TCT AGA TCT TCC CAG TCG YTG GAG GAG TCC GGG 3′
		RSCVH4	5′ GGT GGT TCC TCT AGA TCT TCC CAG SAG CAG CTG RTG GAG TCC GG 3′
	VH 3′ Reverse	RSCG-B	5´ CCT GGC CGG CCT GGC CAC TAG TGA CTG AYG GAG CCT TAG GTT GCC C 3′
Overlap	Overlap Forward	RSC-F	5′ GAG GAG GAG GAG GAG GAG GCG GGG CCC AGG CGG CCG AGC TC 3´
	Overlap Reverse	RSC-B	5′ GAG GAG GAG GAG GAG GAG CCT GGC CGG CCT GGC CAC TAG TG 3′