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. 2018 Mar 6;54(3):216–227. doi: 10.1007/s11627-018-9893-2

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Figure 1. — Schematic representation of the constructs for SSI and bar gene expression. (A) Construction map of pAHC17-SSI expression region. It contained the maize ubiquitin 1 promoter (Ubi1), the rice SSI gene, and nopaline synthase terminator (Nos-ter). The SSI ORF was sub-cloned into pAHC17 vector using BamHI restriction site. (B) Construction map of pJL10P5-SSI expression region. It contained Dy10 high-molecular-weight glutenin promoter (HMW-GS Dy10), the rice SSI gene, and the Dx5 terminator. The SSI ORF was sub-cloned into pJL10P5 using Pme I restriction site. (C) Construction map of pAHC20 containing bar gene which confers resistance to the herbicide Liberty® (glufosinate-ammonium).