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. 2018 Mar 6;54(3):216–227. doi: 10.1007/s11627-018-9893-2

Figure 3.

Figure 3.

Southern blot analysis of three T2 generation transgenic Triticum aestivum L. cv. ‘Bobwhite’ events and a non-transgenic Bobwhite (BW) control. Twenty-five micrograms of leaf DNA was digested with BamHI for DY10, or HindIII for Ub1 and Ub2 events, fractioned on 0.8% agarose gel, transferred to a nylon membrane, and hybridized with 32p-labeled PCR product of rice SSI gene. Ub1, event number 1286 with ubiquitin promoter; Ub2, event number 1700 with ubiquitin promoter; Dy10, event number 2173 with Dy10 promoter; BW, non-transgenic Bobwhite variety. Arrows indicated the endogenous SSI genes in hexaploid wheat.