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. 2018 May 9;9:1005. doi: 10.3389/fimmu.2018.01005

Figure 1.

Figure 1

Expression of Sema-3e transcripts in dendritic cell (DC) and natural killer (NK) cells. Immature DCs (iDCs) were generated from BALB/c mice bone marrow by culturing the cells with GM-SCF for 7 days. DC were stimulated either by lipopolysaccharide (LPS) (1 µg/ml) or Poly:IC (PIC, 20 µg/ml) for 12 h. NK cells were sorted from the spleen of BALB/c mice, either used directly as resting NK cells (rNK) or cultured in the presence of IL-2 (1,000 U/ml) for 4 days (aNK). Sema-3e−/− iDC and Sema-3e−/− NK were used as negative controls. 4T1breast cancer cell line was used as a positive control. The levels of Sema-3e were quantified using RT qPCR (A,B). (A) The expression of Sema-3e in unstimulated iDC or DC stimulated by either LPS or PIC. (B) The expression of Sema-3e in rNK and IL-2 activated NK cells. One-way ANOVA followed by multiple comparison test was applied to compare between different groups at significance level of 0.05, n = 3/group. Two-tailed Student’s t-test was used to compare between two different groups at significance level of 0.05. Each experiment was carried out at least three times.