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. 2018 May 9;9:1005. doi: 10.3389/fimmu.2018.01005

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Copyright © 2018 Alamri, Rahman, Zhang, Alamri, Gounni and Kung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intracellular and surface expression of semaphorin-3E (Sema-3E) proteins in dendritic cell (DC) and natural killer (NK) cells. Intracellular and/or surface expression of Sema-3E proteins on DC (A) or NK cells (B) were examined by APC anti-Sema-3E mAb in flow cytometry. Cell preparations of immature DC, DC stimulated by lipopolysaccharide (LPS) (DC + LPS) or DC stimulated by PIC (DC + PIC) either from Sema-3E^−/− (red line) or Sema-3E^+/+ (blue line) mice were used. Sema-3E^−/− or Sema-3E^+/+ resting NK (rNK) and IL-2 activated NK cells (aNKs) were used. NK or DC cells from Sema-3E^−/− mice were used as negative controls. 4T1 breast cancer cell line was used as a positive control (C). Unstained (red line) and stained Sema-3E^−/− aNK cells (blue line) were used for Sema-3E mAb specificity (C) (n = 3 independent experiments).

Intracellular and surface expression of semaphorin-3E (Sema-3E) proteins in dendritic cell (DC) and natural killer (NK) cells. Intracellular and/or surface expression of Sema-3E proteins on DC (A) or NK cells (B) were examined by APC anti-Sema-3E mAb in flow cytometry. Cell preparations of immature DC, DC stimulated by lipopolysaccharide (LPS) (DC + LPS) or DC stimulated by PIC (DC + PIC) either from Sema-3E^−/− (red line) or Sema-3E^+/+ (blue line) mice were used. Sema-3E^−/− or Sema-3E^+/+ resting NK (rNK) and IL-2 activated NK cells (aNKs) were used. NK or DC cells from Sema-3E^−/− mice were used as negative controls. 4T1 breast cancer cell line was used as a positive control (C). Unstained (red line) and stained Sema-3E^−/− aNK cells (blue line) were used for Sema-3E mAb specificity (C) (n = 3 independent experiments).