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. 2018 May 9;9:1005. doi: 10.3389/fimmu.2018.01005

Figure 3.

Figure 3

Expression of Plexin D1 and NRP1 receptors in dendritic cell (DC) and natural killer (NK) cells. Immature DCs (iDCs) were generated from BALB/c mice bone marrow by culturing the cells with GM-SCF for 7 days. DC were stimulated either by lipopolysaccharide (LPS) (1 µg/ml) or Poly:IC (PIC, 20 µg/ml) for 12 h. NK cells were sorted from the spleen of BALB/c mice, either used directly as resting NK cells (rNK) or cultured in the presence of IL-2 (1,000 U/ml) for 4 days (activated NK cell). The levels of Plexin D1 and NRP1 were quantified using RT qPCR (A,B). (A) The expression of Plexin D1 and NRP1 in unstimulated iDC or DC stimulated by either LPS or PIC. (B) The expression of Plexin D1 and NRP1 in rNK cells and IL-2 activated NK cells. RNase-free water used as negative specificity control. One-way ANOVA followed by multiple comparison test was applied to compare between different groups at significance level of 0.05, n = 3/group (A). Each experiment was carried out at least three times. Two-tailed Student’s t-test was used to compare between different groups at significance level of 0.05 (n = 3 independent experiments) (B).