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. 2018 May 9;9:1005. doi: 10.3389/fimmu.2018.01005

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Copyright © 2018 Alamri, Rahman, Zhang, Alamri, Gounni and Kung.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Intracellular and surface expression of Plexin D1 and NRP1 receptor proteins in dendritic cells (DC) and natural killer (NK) cells. Intracellular and/or surface expression of Plexin D1 and NRP1 receptor proteins on DC (A,B) or NK cells (C,D) were examined by respective APC anti-Plexin D1 and PE anti-NRP1 mAbs in flow cytometry. Semaphorin-3E (Sema-3E)^+/+ cells preparations of immature DC, DC stimulated by lipopolysaccharide (LPS) (DC + LPS) or DC stimulated by PIC (DC + PIC) were used (blue line). Sema-3E^+/+ resting NK (rNK) and IL-2 activated NK cells (aNK) were used (blue line). Isotype control mAbs (IgG2b for Plexin D1, IgG2a for NRP1) were used as negative controls (red line). (A) Expression of Plexin D1 on DC. (B) Expression of NRP1 on DC. (C) Expression of Plexin D1 on NK. (D) Expression of NRP1 on NK (n = 3 independent experiments).

Intracellular and surface expression of Plexin D1 and NRP1 receptor proteins in dendritic cells (DC) and natural killer (NK) cells. Intracellular and/or surface expression of Plexin D1 and NRP1 receptor proteins on DC (A,B) or NK cells (C,D) were examined by respective APC anti-Plexin D1 and PE anti-NRP1 mAbs in flow cytometry. Semaphorin-3E (Sema-3E)^+/+ cells preparations of immature DC, DC stimulated by lipopolysaccharide (LPS) (DC + LPS) or DC stimulated by PIC (DC + PIC) were used (blue line). Sema-3E^+/+ resting NK (rNK) and IL-2 activated NK cells (aNK) were used (blue line). Isotype control mAbs (IgG2b for Plexin D1, IgG2a for NRP1) were used as negative controls (red line). (A) Expression of Plexin D1 on DC. (B) Expression of NRP1 on DC. (C) Expression of Plexin D1 on NK. (D) Expression of NRP1 on NK (n = 3 independent experiments).