Figure 5.

Recombinant semaphorin-3E (Sema-3E) alone did not affect migrations of activated natural killer (NK)-cell migration in plain mouse culture medium: NK cells were purified and activated with IL-2 for 4 days. Sema-3E was added to plain mouse medium (MM) using at different concentrations (6–200 ng/ml). Plain MM was used as negative control. Conditioned medium from lipopolysaccharide (LPS)-stimulated dendritic cell (DC) was used as positive control. Activated NK-cell migration was assayed in the trans-well chamber. One-way ANOVA was used for comparing data from more than two groups. Two-tailed Student’s t-test was used to compare between different groups at significance level of 0.05. The statistical analysis was obtained from n-value = 3.