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. 2018 May 9;9:1005. doi: 10.3389/fimmu.2018.01005

Figure 6.

Figure 6

Semaphorin-3E (Sema-3E) produced by immature dendritic cell (iDC) modulated activated natural killer (NK)-cell migration: migrations of the IL-2 activated NK cells (aNKs) toward different DC conditioned media were examined in the trans-well migration assay. Conditioned media of the Sema-3E+/+ and Sema-3E−/− iIDC, mature lipopolysaccharide (LPS)-stimulated DC (DC + LPS), mature PIC-stimulated DC (DC + PIC) were collected on day 8 of the DC cultures. (A) NK-cell migrations toward the conditioned media of the Sema-3E+/+ DC. (B) NK-cell migrations toward the conditioned media of the Sema-3E−/− DC. (C–E) Direct comparisons of the aNK migrations in various conditioned media of the Sema-3E+/+ (WT) DC and Sema-3E−/− (KO) DC: (C) conditioned media of the iDC. (D) Conditioned media from the LPS-stimulated DC. (E) Conditioned media from the Poly I:C-stimulated DC. One-way ANOVA was used for comparing data from three or more groups (A,B) and two-tailed Student’s t-test was used to compare between two different groups at significance level of 0.05, n = 3/group (C,D,E). Each experiment was carried out at least three times.