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. 2018 May 9;9:1005. doi: 10.3389/fimmu.2018.01005

Figure 7.

Figure 7

Blocking of semaphorin-3E (Sema-3E) produced by immature dendritic cell (iDC) enhanced activated natural killer (NK) cells migration: migrations of the IL-2 activated NK cells toward the conditioned medium of the Sema-3E−/− (KO IDC) and Sema-3E+/+ (WT IDC) iDC were assayed. (A) Recombinant Plexin D1 (100 ng/ml) was added to the WT IDC conditioned medium at the time of running the migration assay. 1% PBS and 1% BSA (100 ng/ml) were used as negative controls. Conditioned medium of the Sema-3E−/− (KO IDC) was used as positive control. (B) The introduction of recombinant Sema-3E back to the conditioned medium of the Sema-3E−/− iDC abrogated the enhanced NK-cell migration. Recombinant Sema-3E (50 ng/ml) was added to the KO IDC conditioned medium at the time of running the migration assay. (C) Sema-3E has no regularity effect on NK-cell migration through CXCL12 or CXCL10 chemokines. CXCL12 chemokine (50 ng/ml) and CXCL10 chemokine (100 ng/ml) were added to mouse medium at the time of running the migration assay. Statistical significance was established by one-way ANOVA was obtained to establish the comparison between three or more groups (A,C). Two-tailed Student’s t-test was used to compare between two indicated groups at significance level of 0.05 (B,C) (n = 3 independent experiments).