Table 2.

PCR primers and conditions.

Gene	Primer sequence (5′ → 3′)	Size of PCR product (bp)	Amplification profile
tph1a	F: TTCAAGGACAATGTCTATCG	214	94°C—30 s
	R: GGGAGTCGCAGTGTTTGATG		55°C—30 s
	Genebank Acc. No.: AF548566 (46)		72°C—60 s (35 cycles)
tph1b	F: TACCTGCAGAACCTGCCTCT	430	94°C—30 s
	R: AGAGAAGACCAGCCCCGTAT		55°C—30 s
	Genebank Acc. No.: BC154120 (46)		72°C—60 s (35 cycles)
tph2	F: GTGTGAACTCCAAAGCAGCA	684	94°C—30 s
	R: TGGTATTCCTTCCCCATCTG		55°C—30 s
	Genebank Acc. No.: AB125219 (46)		72°C—60 s (35 cycles)
cyp19a1b (AroB)	F: TTAAAGAGGTGTGTCTGTATGTGAGGTG	1,435	42°C—10 min
	R: GGAATTTACTCTGTGCGCCTTTAAATGT		94°C—30 s
	Genebank Acc. No: BC076104		60°C—15 s
			72°C—90 s (40 cycles)
β-Actin	F: GGTATGGGACAGAAAGACAG	330	94°C—30 s
	R: AGAGTCCATCACGATACCAG		58°C—30 s
	Genebank Acc. No: AF025305 (3)		72°C—60 s (34 cycles)