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. 2018 May 9;9:1009. doi: 10.3389/fimmu.2018.01009

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Copyright © 2018 Easom, Stegmann, Swadling, Pallett, Burton, Odera, Schmidt, Huang, Fusai, Davidson and Maini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IL-15 restores NK cell function following PLC/PRF/5 coculture. (A) Diagram showing experimental design of PLC/PRF/5 coculture followed by rest in fresh media ± cytokine before K562 challenge. (B) CD107a staining following K562 challenge after PLC/PRF/5 coculture, then rest overnight in fresh media ± the indicated cytokine using intrahepatic NK cells isolated from a liver explant (Ex) and from a liver perfusate (Perf). (C) Intracellular IFNγ staining following K562 challenge after PLC/PRF/5 coculture, then rest overnight in fresh media ± the indicated cytokine using intrahepatic NK cells isolated from a liver explant (Ex) and from a liver perfusate (Perf). (D) Summary data showing CD107a staining following K562 challenge of intrahepatic NK cells unstimulated, after PLC/PRF/5 coculture, after PLC/PRF/5 coculture then rest in fresh media and after PLC/PRF/5 coculture then rest in IL-15 containing media (n = 5 liver samples). (E) Summary data showing intracellular IFNγ staining following K562 challenge of intrahepatic NK cells unstimulated, after PLC/PRF/5 coculture, after PLC/PRF/5 coculture then rest in fresh media and after PLC/PRF/5 coculture then rest in IL-15 containing media (n = 5 liver samples). (F) Example of CD107a staining on paired intrahepatic and tumor-infiltrating NK cells following K562 challenge after overnight rest with IL-15. Groups were compared using unpaired t test with Welch’s correction. p ≤ 0.05 was considered to be significant for all tests. Figures are labeled: *p ≤ 0.05.