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. 2018 Apr 30;54(3):240–252. doi: 10.1007/s11627-018-9905-2

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Figure 4. — Rapid development of single somatic embryos of Zea may L. on the surface of recently transformed zygotic immature maize embryos shown under bright field microscopy for Fast-Flowering Mini-Maize at 7 d after infection (A). Green fluorescence of somatic embryos at progressive durations after Agrobacterium tumefaciens infection are shown for pioneer inbred PH184C (at 4 d (B), 7 d (C), and 10 d (D) after infection ). E, F Germinating transgenic plantlets using the public inbred Mo17 at 14 and 24 d (respectively) after A. tumefaciens infection showing 1–2 plantlets developing from each of the originally infected immature embryos