Table 1.

Genetic components used to construct expression cassettes within the T-DNAs

Component type	Label	Description	References
Promoters	Ubi pro	The maize ubiquitin promoter, the 5′ UTR, and the first intron	Christensen et al. (1992)
	Sb-ALS pro	The sorghum ALS promoter	SB-ALS promoter and 5′ UTR, DOE-JGI Sbi v3.1, SBChr04, bases 49239164-49240031. DOE-JGI Sbi v3.1 corresponds to Sorghum bicolor BTx623 assembly v3.0.1 and gene annotation v3.1 available from phytozome (http://phytozome.jgi.doe.gov/). Chromosome 4 of Sbi v3.1 is registered as NCBI accessions NC_012873.2 and CM000763.3
	PLTPpro	Maize phospholipid transferase promoter	Anand et al. (2017a, b) US20170121722
	LTP2pro	Barley lipid transferase promoter	Kalla et al. (1994)
	nos pro	The Agrobacterium-derived nopaline synthase promoter	An (1986)
	Axig1pro	The maize Axig1 promoter	Garnaat et al. (2002) WO2002006499
	rab17 pro	The maize rab17 promoter and 5’ UTR	Busk et al. (1997)
3′ Sequences	PINII	The potato proteinase inhibitor II (pinII) 3′ sequence	An et al. (1989)
	Sb-PEPC 3′	The PepCarboxylase 3′ regulatory sequence from Sorghum bicolor	Anand et al. (2017a, b) US20170121722
	Os-T28 3’	The T28 3′ regulatory sequence from Oryza sativa	Bhyri et al. (2014) US20140130205 A1
Marker genes	PMI	The phosphomannose isomerase gene from E. coli	Negrotto et al. (2000)
	HRA	The maize ALS double mutant gene conferring herbicide resistance	Green et al. 2009
	moPAT	A maize codon-optimized gene encoding phosphinothricin acetyltransferase	Jayne et al. (2000) US6096947
	ZsYELLOW	The ZsYellow1 N1 gene encoding a yellow fluorescent protein from Zoanthus sp.	Matz et al. (1999)
	ZsGREEN	A gene encoding the ZsGREEN protein from Zoanthus sp.	Matz et al. (1999)
Maize morphogenic genes	Zm-Wus2	The maize Wuschel2 (Wus2) gene	Lowe et al. (2007) US7256322B2
	Zm-Bbm	The maize Baby boom gene (Bbm)	Gordon-Kamm et al. (2005) WO2005075655