Table 3.

Summary data for transformation experiments for the pioneer Zea may L. inbred lines PHR03, PHH5G, and PH1V69 after transformation with a T-DNA containing Zm-PLTP_pro::Bbm plus either Nos_pro::Wus2 (PHP79065) or Zm-Axig1::Wus2 (PHP79066), showing the number of immature embryos infected with Agrobacterium tumefaciens, the resulting number of T0 plants sampled for PCR, the transformation frequency ((number T0 plants ÷ initial number of Embryo) × 100), the number of plants that were single copy for the transgenes (quality events based on qPCR) and the final frequency of usable quality events (single copy with no backbone relative to the original number of starting immature embryos). For each inbred, replicates are indicated in separate rows, with each row reflecting immature embryos harvested from a single ear

Genotype	Plasmid	Embryo Number	Number of Plants sampled	Transformation Frequency (%)	Plant QE Number	%UQE
PHR03	79065	210	60	28.6	10	4.8
PHR03	79066	200	125	62.5	25	12.5
PHH5G	79065	162	41	25.3	11	6.8
PHH5G	79066	165	15	9.1	6	3.7
PH1V69	79065	148	37	25	9	6
PH1V69	79066	155	47	30.3	14	9