Fig. 7.
DUSP6 overexpression in allogeneic T cells attenuates the development of GVHD without compromising graft-versus-tumor effects. a–e C57BL/6 mice-derived CD3+ T cells were transduced with DUSP6-ΔNGFR or ΔNGFR, and the isolated ΔNGFR+ T cells (1 × 105 T cells per mouse) were transplanted into sublethally irradiated Balb/c mice together with A20 expressing EGFP-luciferase (A20-GL). b The frequency of donor T cells in the peripheral blood (n = 8 mice for each group, unpaired two-sided t-test). c The sequential monitoring of the body weight. d Total photon counts measured by in vivo bioluminescence imaging. e Kaplan–Meier analysis for overall survival (e, log-rank test). Representative data of two independent experiments. f C57BL/6 mouse-derived CD3+ T cells were transduced with DUSP6-ΔNGFR or ΔNGFR, and a mixed population of ΔNGFR-positive and negative cells (3 × 105 cells per mouse) and A20-GL were transplanted into sublethally irradiated Balb/c mice. g The frequency of ΔNGFR+/− donor T cells was analyzed in peripheral blood (unpaired two-sided t-test). h The frequency of ΔNGFR+ T cells within the H-2Kb+ donor T-cell population was monitored at the indicated time points. (i) The frequency of CD8+ T cells within the ΔNGFR+ donor T-cell population was analyzed (unpaired two-sided t-test for each time point). In g–i, n = 9 for the control-day 9, n = 3 for the control-day 12, n = 8 for the DUSP6-day 12, n = 2 for the DUSP6-day 20, and n = 10 for the other plots. j At the time of death, the relative abundance of ΔNGFR+/− cells within the H-2Kb+ donor T-cell population was analyzed in the spleen (n = 8 for DUSP6-T cells and n = 10 for control T cells, unpaired two-sided t-test). NS, not significant. Horizontal lines indicate the means ± s.d