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. 2018 May 9;9:447. doi: 10.3389/fphar.2018.00447

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Copyright © 2018 Liu, Yang, Mu and Fu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of EGCG on DEX-induced differentiation and 11β-HSD activity in primary osteoblasts. (A) Mineralization of cells was inhibited by DEX, but pretreatment with EGCG reduced this effect. (B) Expression levels of representative osteogenic differentiation transcriptional factors (Runx2, BMP-2, and Osterix) were analyzed by real-time PCR. (C) Expression of 11β-HSD and Runx2 was measured by Western blot, and quantitated levels of protein were statistically evaluated, as shown in the column. The data were expressed as the mean ± SD. ^##P < 0.01, ^###P < 0.001 vs. control and ^∗∗P < 0.01, ^∗P < 0.05 vs. DEX.