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. 2018 May 9;9:1020. doi: 10.3389/fimmu.2018.01020

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Copyright © 2018 Montero-Herradón, García-Ceca and Zapata.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Medullary TECs (mTEC) subsets defined by the expression of UEA1/MHCII cell markers in E17.5, 7PN, and adult thymi in both wild type (WT) and EphB-deficient mice. (A) Dot plots show mTEC^lo (MHCII^medUEA1⁺) and mTEC^hi (MHCII^hiUEA1⁺) cell subpopulations according to MHCII and UEA1 expression, gated in total EpCAM⁺CD45⁻ cells. Numerical values show the frequency of each population. (B,C) Figures show the proportions of mTEC^lo (B) and mTEC^hi cells (C). The significance of the Student’s t-test probability is indicated: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.005; and ^#p ≤ 0.05 between mutants; ns, non-significant.