Table 2.
Plasmids used in this study.
| Plasmid | Characteristics | Source |
|---|---|---|
| pZMP | Constitutive E. coli – C. glutamicum shuttle expression vector | Walter et al., 2016 |
| pZMP::relCg | pZMP carrying the gene rel (cg1861) from C. glutamicum CR099 | This study |
| pZMP::relSCg | pZMP carrying the gene relS (cg2324) from C. glutamicum CR099 | This study |
| pZMP::relHCg | pZMP carrying the gene relH (cg1485) from C. glutamicum CR099 | This study |
| pK18mobsacB | Suicide vector for gene deletion by homologous recombination | Schäfer et al., 1994 |
| pK18mobsacB_relHCg | pK18mobsacB carrying the up- and downstream region (500 bp, respectively) of the gene relH from C. glutamicum CR099 | This study |
| pTXB1 | E. coli expression vector, carrying the self-cleavable Mxe intein/chitin binding domain | New England Biolabs |
| pTXB1::relCg | pTXB1 carrying the gene rel (cg1861) from C. glutamicum CR099 | Ruwe et al., 2017 |
| pTXB1::relSCg | pTXB1 carrying the gene relS (cg2324) from C. glutamicum CR099 | Ruwe et al., 2017 |
| pTXB1::relHCg | pTXB1 carrying the gene relH (cg1485) from C. glutamicum CR099 | This study |