Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 15;8:7583. doi: 10.1038/s41598-018-25922-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Registration precision. (a) A 3D-SIM image of multicolor beads immobilized both on the coverslip and on the glass slide before (left) and after (right) registration. Maximum projections of the quarter field of view (20 × 20 × 8 µm) along the XY plane and the XZ plane are shown with a region magnified in the inset. The blue, green, and orange channels are indicated by their respective central emission wavelengths. Scale bars represent 5 µm for the larger field of view including the vertical (Z) axis and 1.25 µm for the inset. (b) Mean distances of individual bead localizations in the blue and orange channels relative to the green reference channel, applying the ‘global’ and ‘global + local’ registration methods. The 3D positions of 527 beads on two-layer multicolor bead samples were determined by 3D Gaussian fitting, and the 3D distances were averaged. Error bars indicate SD. (c) Plots of beads along the X coordinate. The Pearson correlation coefficient (r) is shown for each channel to show the correlation, if any, between deviation and position in the field of view.