Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 15;8:7583. doi: 10.1038/s41598-018-25922-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Possible chromatic shifts in calibration slides. (a) Translation along the Z-axis (T_Z) measured for the different refractive indices of immersion oils using the blue channel as a reference. Two-layer multicolor bead slides were imaged by 3D-SIM using immersion oils with different refractive indices as indicated. Bars represent the standard deviation (SD) of 1–3 measurements at different positions of the two slides. The Abbe numbers (v_D) for these immersion oils are also shown in parenthesis. (b) The same data in (a) shown for each individual parameter. Larger values indicate greater fluctuation. (c) A single set of four two-layer blue bead slides (a–d) imaged with different objective lenses in the blue, green, and orange channels by deconvolution microscopy; the blue channel was used as a reference to measure T_Z. Bars represent SD from measurements taken at 5–6 positions of each coverslip. (d) Translation along the Z axis measured for three coverslips (numbered as 1, 2, and 3) from different companies. All coverslips were of standard thickness No. 1.5 (0.16–0.19 mm) except for Zeiss No. 1.5 H (0.170 ± 0.005 mm) and Fisher No. 1 (0.13–0.17 mm). Multicolor beads were imaged by 3D-SIM using a silicone immersion objective lens, except for coverslips from Ibidi, for which the results from deconvolution are shown since beam polarization (required for 3D-SIM) was affected by these plastic coverslips. Error bars represent SD from 3–7 acquisitions at different positions on each slide. (e) Translation of the orange channel position relative to the blue channel (detected on separate cameras) monitored over eight months. Error bars (horizontal lines) indicate the negligible SD of sample measurements within the same day. (f) T_Z measured for beads and biological samples using silicone immersion objective lens observed in the blue (442 nm) and green (525 nm) channels. “Cells” indicates fixed biological samples on the coverslip imaged by 3D-SIM at a mean observation depth of ~3 µm. Bars represent SD from at least three different slides.