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. 2018 May 15;8:7583. doi: 10.1038/s41598-018-25922-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chromatic shifts of replicates on the same coverslip. (a) Illustration of a chambered coverglass with numbers temporarily assigned to individual chambers. (b) Multispectral fluorescent beads and fixed cells stained with phalloidin conjugated with Alexa 488 and 594 were imaged with 3D-SIM. Then, the vector sum of the global alignment parameters for green and red was compared with chamber No. 1. (c) Mean difference of the vector sum of the global alignment parameters with chamber No. 1 (“Total”), neighboring chambers (“Neighbor”), and chambers with no contact (“No contact”).