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. 2018 May 15;8:7550. doi: 10.1038/s41598-018-25245-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Segmentation and tractography of the corpus callosum (CC) subregions. (A) Representative image of CC segmentation on a mid-brain slice on sagittal orientation divided into 30 vertical segments of equal width. (B) Relabeling of the 30 segments into seven CC subregions. Because the smallest subregion, the rostrum (red), was not fully developed/visible in all infants, this structure was combined with the genu. (C) Probabilistic tractography of the genu (blue), rostral body (pink), anterior midbody (burgundy), posterior midbody (light green), isthmus (green), and splenium (red) overlaid on a diffusion B0 image in axial orientation in a 29-week gestational age preemie imaged at 30 weeks postmenstrual age. (D,E,F) Tractography in the same 29 weeks preterm infant imaged at 33, 38, and 53 weeks postmenstrual age, respectively.