FIG 5.

miR-199a-5p regulates the intracellular localization and glycosylation of TGN46. (A) Steady-state intracellular location of TGN46 in HeLa cells transfected with control mimic (CM) and miR-199a-5p for 48 h and costained with GM130. Scale bar = 25 μm. Magnif, magnification of areas in dashed boxes. Scale bar = 10 μm. Right, colocalization coefficients for GM130 and TGN46. Data are from three independent experiments and are expressed as mean values ± SEM. (B) Representative Western blot analysis of TGN46 in HeLa cells transfected with CM or miR-199a-5p mimic after 48 h. MW, molecular weight in thousands.