FIG 5.

Ectopically expressed HuR rescues the epithelial barrier function in α4-silenced cells. (A) Representative immunoblots of claudin-1, claudin-3, ZO-1, and E-cadherin in α4-deficient cells with or without HuR overexpression. The cell lysates were harvested 48 h after the cells were transfected with C-siRNA or cotransfected with siα4 and recombinant adenoviral plasmids containing HuR (AdHuR) or control adenoviral vector (Adnull). (B) Changes in the epithelial barrier function as indicated by changes in TEER and FITC-dextran paracellular permeability in the cells shown in panel A. The values are the means and SEM of data from six samples. *, P < 0.05 compared with C-siRNA-transfected cells or cells cotransfected with siα4 and AdHuR.