FIG 1.
(A) A schematic diagram shows the distinct mechanisms used by multiple small molecule cap-dependent inhibitors to inhibit eIF4E function and cap-dependent translation initiation. (B and C) 4EGI-1 induces p53 in LNCaP cells. LNCaP cells were treated with 10 μM etoposide, 50 μM 4EGI-1, and 50 μM 4Ei-1 for 2 h (B) or 24 h (C). After treatment, cells were lysed. p53 and β-actin were detected following SDS-PAGE and Western blotting. (D) p53 induced by 4EGI-1 is accompanied by increased expression of PUMA. Subconfluent LNCaP cells were treated with 50 μM 4EGI-1 for the indicated time periods. After treatment, the cells were lysed. p53, PUMA, p21Cip1, and β-actin were detected following SDS-PAGE and Western blotting. The results in panels B to D are representative of three individual experiments.