Figure 7. Cellular changes of the asthmatic mouse lung due to MIDD0301 treatment.

Representative images of lungs from mice that were injected i.p. with EdU, a thymidine analog, and harvested four hours later. After standard histology processing, sections were treated with a fluorescent azide under “Click” chemistry conditions enabling conjugation of incorporated EdU to visualize cells that underwent the S phase during a four hour period (column 1). Slides were counterstained with Hoechst 33342 (column 2) and superimposed images are presented in column 3. Row 1 presents lung images of control mice. Row 2 depicts lung images of vehicle-treated ova s/c mice and row 3 images of MIDD0301 (100 mg/kg) treated ova s/c mice.