FIG 4 .

Effects of mutant alleles on exponential growth rates in different genetic backgrounds. (A to M) Exponential growth rates of 10 strains with differing genetic distances carrying the indicated mutant allele relative to the respective wild-type strain. All values represent the means of a minimum of four biological replicates and two technical replicates with error bars representing the standard deviations. (N) Absolute fitness difference (as a percentage) of all mutant constructs compared to the corresponding mutants in S. Typhimurium LT2. The values were obtained by subtracting the relative growth rate of a mutant in any given strain with the relative growth rate of S. Typhimurium LT2 carrying the identical mutation. For complete strain designations and phylogenetic relationships, see Fig. 3 and Table S1.