FIG 5 .

Effects of mutant alleles on antibiotic resistance in different genetic backgrounds. (A to M) MICs of the different antibiotics for each strain carrying the indicated mutant allele and the respective wild-type strain. Light gray bars indicate the MIC of the wild-type strain, and dark gray bars represent the MIC of the mutant. Dashed lines indicate the detection limit. Two MICs for fusA P413L could not be determined due to strain construction difficulties (S. Indiana) or lethality on plates containing EDTA (E. coli fusA P413L). (N) Fold change difference of mutations introduced in the indicated strain over the fold change difference that the mutation causes in S. Typhimurium LT2. For complete strain designations and phylogenetic relationships, see Fig. 3 and Table S1. Abbreviations: RIF, rifampin; CIP, ciprofloxacin; STM, streptomycin; ACT, actinonin; FUS, fusidic acid; TGC, tigecycline; COL, colistin; ETP, ertapenem; MEC, amdinocillin; ND, not determined.