TABLE 3 .
Primers and qPCR assays used in this study
| Primer or assay | Sequence (5′→3′) | Reference(s) |
|---|---|---|
| Primers | ||
| 2 f | TATCCCAGTTCAATATTTCTCCACTACACC | 30 |
| 2 r | ACACAAAATATAGGCAGAGAGAGACTACT | |
| 4 f | CTGTTACTTGTTCTGGACTCTCGATAATTGG | 30 |
| 4 r | GCCCACTCCTGTTAAAATCCTACCCGCATTG | |
| JVS99L | TTGCCAGCAGAATTGCAGCA | This study |
| JVS100R | AAATAGGTATCTCCTTCCACC | This study |
| JVS101L | CTGCTGGGGTACTAACAGGG | This study |
| JVS102R | CGGCACTTCGATGTGAATGG | This study |
| JVS103L | ATCTTGACAAGCAAGGGAAAAT | This study |
| JVS104R | TTCCTTATGCTTTTGGACGTTT | This study |
| PCR assays | ||
| Serotype 2 Fwd | TTATGGACTGGCTGATGGTTCTC | 22, 58 |
| Serotype 2 Rev | AAATCCTGACCCAATAATAGCCTTT | |
| Serotype 2 probea | AGGTCAACGTATTGGAACTCTTAGAAATTGGGAAA | |
| Serotype 4 Fwd | TGGGATGACATTTCTACGCACTA | 22, 58 |
| Serotype 4 Rev | CCGTCGCTGATGCTTTATCA | |
| Serotype 4 probea | TCCTATTGGATGGTTAGTTGGTGA | |
| Serotype 19F Fwd | GGTCATGCGAGATACGACAGAA | 22, 58 |
| Serotype 19F Rev | TCCTCATCAGTCCCAACCAATT | |
| Serotype 19F probea | ACCTGAAGGAGTAGCTGCTGGAACGTTG |
a
Probes are labeled 5′ with 6-carboxyfluorescein (FAM) and 3′ with black hole quencher 1 (BHQ1).