FIG 3 .
Schematic diagram of the H. pylori J166 cagPAI in the WT and selected deletion mutants. In J166 ΔcagY, the entire cagY gene is replaced by a cat-rpsL cassette (coding for streptomycin susceptibility and chloramphenicol resistance). The ΔcagYMRR mutant has an unmarked, in-frame deletion of the MRR created by contraselection. In J166 cagXY, cag1 to 6 are replaced with cat, and cag9 to 25 are replaced with a kanamycin resistance cassette, starting from after the putative cagY promoter in cag9. J166 cagY has an unmarked deletion of cag1 to 6 and cagX, while cag9 to 25 downstream of the cagX/Y promoter in cag9 are replaced with a kanamycin resistance cassette. cagA is intact in all strains since it is not on the cagPAI in J166 (56).