Figure 3.

2D ¹³C-¹³C correlation DQ/SQ spectra of specifically ¹³C-labeled (a) α-syn monomers, and α-syn monomers mixed with nanodiscs in (b) 2:1, (c) 8:1, and (d) 16:1 protein-to-nanodisc molar ratio. For one-dimensional projections (black lines in e–h), the Cα-Cβ crosspeak region of valines of the 2D spectra was summed up. The projections were deconvoluted using Gaussian line shapes with the help of the DMfit program. The projections are given in black, the single deconvoluted peaks in gray, the resulting fitting curve is shown in red for each sample, and the difference spectrum is given in green. The simulations were done for the monomeric form (e); for α-syn monomers mixed with nanodiscs in a protein-to-nanodisc molar ratio of (f) 2:1, (g) 8:1, and (h) 16:1; and for the fibrillar form (Fig. S2).