Figure 6.

Relative Ca²⁺ binding free energy. The change in Ca²⁺ binding free energy is normalized to the average of WT cTnI, plotted as ΔΔ $G_{bind}^{wild\to mut}$. Four representative frames from all simulations for a given isoform were calculated and averaged; error bars are ±SE. Mean ± SE are presented. ^∗p < 0.05 from a Student’s t-test to the WT HSD value.