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. 2018 Apr 10;114(7):1680–1694. doi: 10.1016/j.bpj.2018.02.019

Figure 3.

Figure 3

Vinculin dynamics are strongly affected by the ability of vinculin to bind talin, but not actin. (A) Representative FAs of cells expressing the VinTS and its mutant variants (VinTS A50I, VinTS I997A) are displayed in the acceptor channel during FRAP imaging. Scale bars, 3 μm. (B) Vinculin FRAP recovery curves are shown for cells expressing VinTS, VinTS A50I, or VinTS I997A (n = 34, 21, and 18 FAs, respectively, from seven independent experiments). (C) Box-whisker plots are given of the half-time of recovery for the FAs that were analyzed in (B). (D) Box-whisker plots are given of the mobile fraction for the FAs that were analyzed in (B). Differences between groups were detected using the Steel-Dwass test (∗∗p < 0.01, ∗∗∗p < 0.001); p values for all comparisons can be found in Table S2.