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Splenic plasmacytoid dendritic cell (pDC), macrophages (Mϕ), and polymorphonuclear neutrophil (PMN) distribution during the course of chronic graft-versus-host disease (cGVHD) in B6 and B6.Sle2c2 mice. (A) Gating strategy used to identify pDCs (top), Mϕ (middle), and PMN (bottom panel) from live cells. Frequency (B) and numbers (C) of the gated populations at the indicated time points after cGVHD induction (N = 5–12 per time point, *p < 0.05; **p < 0.01, ***p < 0.001).
