Figure 9.

CD8α⁺ dendritic cells (DCs) are sufficient to induce resistance against chronic graft-versus-host disease (cGVHD). cGVHD was induced in indicated mice for 21 days. A sub-group of B6.Sle2c2 was depleted with anti-CD8a depleting antibody (300 µg/mouse/i.p.) every 3 days and until day 17 while a sub-group of B6.Batf3^−/− mice was i.v. transferred with 50–70 × 10³ CD8α⁺ DC on days 2, 7, and 14 post-cGVHD induction. (A) Representative FACS plots of spleen DC subsets within each group. (B) Frequencies of CD8α⁺ DC among spleen DCs, anti-dsDNA IgG titers, and regulatory T cells (Treg) in CD4⁺ Tcells at day 21 post-cGVHD induction (N = 2–9 mice/group, *p < 0.05; **p < 0.01).