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. Author manuscript; available in PMC: 2018 May 16.
Published in final edited form as: ACS Appl Mater Interfaces. 2018 Mar 6;10(11):9269–9279. doi: 10.1021/acsami.7b18653

Figure 8.

Figure 8

CAP exposure reduces protein synthesis in cancer cells. Cells were treated with 3.16 kV CAP for 60 s and compared to the untreated (no CAP) control condition. The Click-iT Plus OPP Alexa Fluor 647 Protein Synthesis Assay Kit was used to measure protein synthesis. Fluorescence intensities of 50 MDA-MB-231 breast cancer cells and 50 U87 glioblastoma cells from two experiments were quantified. Subcellular distribution of Click-iT Plus OPP staining (green) was revealed by confocal microscopy (a,b,d,e). Expression of OPP decreased between 12 and 24 h in MDA-MB-231 cells (c) and between 6 and 24 h in U87 cells (f). A decrease in pixel intensity correlates with a decrease in the level of protein synthesis. White arrows in (b,e) indicate the loss of OPP (green) in cells. The cell nucleus is visualized with DAPI in blue. Scale bar (bottom image): 10 μm.