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. 2018 May 9;8:137. doi: 10.3389/fcimb.2018.00137

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Copyright © 2018 Lee, Miller, Wu, Casella, Hasegawa and Lamont.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mfa1 fails to polymerize in P. gingivalis strains expressing substitutions in hydrophobic domains. P. gingivalis strains 33277, Δmfa1, or Δmfa1 complemented with pT-COW containing mfa1 wild type allele or the N or C terminal alanine or aspartic acid substitutions were treated at 60° or 100°C prior to SDS-PAGE and immunoblotting with antibodies to (A) Mfa1 or (B) FimA. Results are representative of 4 biological replicates.