Figure 9.

Relative protein expression. (a) represents the specific grouping situation and Western blot result of GAPDH, MyD88, p-p38, p-IκB, and p-JNK1 + JNK2 + JNK3. (b), (c), (d), and (e) represent the relative expression of p-p38, p-IκB, and p-JNK1 + JNK2 + JNK3 according to analysis of gray level differences. Compared with the control group, the p-p38 expression levels in the 100 μg/mL TCP particle group and siRNA-TLR2-1 added group were significant lower (P < 0.001), and they showed, respectively, lower levels than the 100 μg/mL C₂S particle group with and without siRNA-TLR2-1. The positive control group showed high p-p38 expression levels (b). There was a significant difference in the p-IκB levels between the control and 100 μg/mL C₂S particle groups (P < 0.01) as well as the TCP particle group (P < 0.001). Moreover, an obvious difference was observed between the 100 μg/mL C₂S and TCP particle groups (P < 0.01). The LPS induced low p-IκB expression levels (P < 0.01) (c). The 100 μg/mL C₂S particle group expressed high p-JNK1 + JNK2 + JNK3 expression levels (P < 0.01), and they were obviously higher than when siRNA-TLR2-1 was added (P < 0.05). However, the 100 μg/mL TCP + siRNA-TLR2-1 group showed low levels of p-JNK1 + JNK2 + JNK3 expression (P < 0.01) (d). No significant difference in MyD88 levels was observed in the groups, including in the LPS-treated group (e). The data are expressed as the mean ± SD. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 (experimental group versus control). ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 (between experimental groups).