Skip to main content
. 2018 May 7;28(9):1344–1356.e5. doi: 10.1016/j.cub.2018.03.023

Figure 1.

Figure 1

Centromere and Kinetochore Functional Layers Scale Proportionally with Centromere Size

(A) Normal karyotype of female Indian muntjac fibroblasts. Scale bar, 5 μm.

(A′) Centromere length of Indian muntjac chromosomes (box-whisker plots, n = 40 cells, ∼80 kinetochores per chromosome type, Mann-Whitney rank-sum test, p < 0.001 for all comparisons).

(B–G) Immunofluorescence of Indian muntjac chromosome spreads (blue) and the centromere and kinetochore proteins (green) CENP-A (B), Ndc80 (C), CENP-E (D), Mad2 (E), pAurora B (F), and pKNL1 (G). Scale bars, 5 μm.

(B′–G′) Respective quantification of protein levels at Indian muntjac kinetochores, relative to chromosome 3+X (C3X) for CENP-A (B′), Ndc80 (C′), CENP-E (D′), Mad2 (E′), pAurora B (F′), and pKNL1 (G′) (mean ± SD, n ≥ 37 cells per condition, ∼100 kinetochores per chromosome type, ∗∗∗p < 0.001 relative to controls, Mann-Whitney rank-sum test).