REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Human anti - centromere (CREST)	Fitzgerald	Cat#90C-CS1058
Mouse anti - α-tubulin (clone B512)	Sigma-Aldrich	Cat#T5168
Mouse anti - CENP-A	[22]	Lars E.T. Jansen
Rabbit anti - Hec1	Abcam	Cat#ab3613
Mouse anti - cMad2	Santa Cruz	Cat#sc-65492
Rabbit anti - Mad2:	Bethyl Laboratories	Cat#A300-301A
Rabbit anti - pAuroraB, (pT232)	Rockland	Cat#600-401-677
Rabbit anti – AuroraA	Novus Biologicals	Cat#NB100-267
Mouse anti – GAPDH	Proteintech	Cat#60004-1-Ig
Rabbit anti - phospho-histone H3 (S10)	Cell Signaling	Cat#3377
Rabbit anti – survivin	Novus Biologicals	Cat#NB500-201
Rabbit anti - pKNL1 (clone 58A)	[31]	Iain Cheeseman
Sheep anti - CENP-E	[56]	William C. Earnshaw
Rabbit anti - kif4a	Thermo Fisher Scientific	Cat#pa5-30492
Abberior donkey anti-human IgG STAR 580	Abberior Instruments	Cat#D-08-2015Hp
Abberior goat anti- mouse IgG STAR 635p	Abberior Instruments	Cat#S-11-2015Hp
SiR-Tubulin	Spirochrome	Cat#CY-SC002
Chemicals, Peptides, and Recombinant Proteins
Monastrol	Tocris bioscience	Cat#1305
ZM447439 (Aurora B inhibitor)	Selleckchem.com	Cat#S1103
MNL8054 (Aurora A inhibitor)	Selleck	Cat#869363-13-3
AZ 3146 (Mps1 inhibitor)	Tocris bioscience	Cat#3994
GSK923295 (CENP-E inhibitor)	Selleckchem.com	Cat#S7090
BSA 98%	Sigma-Aldrich	Cat#A3294-50G
Experimental Models: Cell Lines
Immortalized female Indian muntjac fibroblasts	[29]	Jerry W Shay
Indian muntjac- H2B-GFP (female)	In this paper	N/A
Indian muntjac- CENP-A-GFP (female)	In this paper	N/A
Oligonucleotides
5′-GCAGACATTGAGAGAATAA-3′ (Ndc80)	Sigma-Aldrich	N/A
5′-GCAGAATGGTTATACAAGT-3′ (Mad2)	Sigma-Aldrich	N/A
5′-GCTGCTGTTGCTGATGCTT-3′ (CLASP1)	Sigma-Aldrich	N/A
5′-GCGTCTCCACGTTTAAGAA-3′ (Survivin)	Sigma-Aldrich	N/A
5′-GAACCGTCAGCAAGACAA-3′ (Kif4a)	Sigma-Aldrich	N/A
5′-GCCAGGGACCTCATTTCAA-3′ (Aurora A)	Sigma-Aldrich	N/A
Recombinant DNA
H2B-GFP	Addgene plasmid # 11680	Geoff Wahl lab
pSV-IRESneo3-CENP-A-EGFP	[57]	Patrick Meraldi
Software and Algorithms
Fiji/ImageJ	ImageJ	N/A
CellProfiler	[58]	CellProfiler (http://cellprofiler.org)
CellProfilerAnalyzer	[58]	CellProfiler (http://cellprofiler.org)
MatLab8.1	The MathWorks	https://www.mathworks.com/products/matlab.html