Figure 4.

Paclitaxel does not decrease the survival of sensory neurons in culture. Sensory neuronal cultures were treated with vehicle (open columns, Veh) or with 10 nM or 300 nM paclitaxel for 5 days (Pac; shaded columns) and then cell viability was measured. As a positive control, vehicle-treated cultures were exposed to H₂O₂ (300 μM) for 1 hour 24 hours prior to analysis of cell survival (shaded columns). The number of annexin V positive cells (A) and propidium iodine (PI) positive cells (B) were counted in a minimum of 5 fields from 3 different harvests and normalized to the total number of neurons in the field. Each column is the mean ± SEM of % of positively stained neurons. An asterisk indicates a significant difference from untreated controls (p<0.05, N=3) using a one way-ANOVA and Bonferroni’s post-hoc test.