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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Mol Cell Neurosci. 2017 Apr 9;82:105–117. doi: 10.1016/j.mcn.2017.04.001

Figure 1.

Figure 1

Paclitaxel decreases PDBu-stimulated CGRP release in cultured sensory neurons. Each column represents the mean ± SEM of basal (white columns) or stimulated (black columns) CGRP release expressed as % of total content. Cultures were exposed to (A) 300 nM or (B) 1 μM paclitaxel for 1, 2, 3 or 5 days prior to stimulation with PDBu (10 nM). An * indicates a significant decrease in PDBu-stimulated release in paclitaxel-treated (300 nM and 1 μM) neurons compared to vehicle-treated neurons (p < 0.05, N = 7–9). (C) Naïve cultures were pre-treated with Bis I (1 μM) for 10 minutes prior to stimulation with PDBu. As a control experiment, naïve cultures were also incubated with 10 nM 4α-PDBu (dotted columns). An * indicates a significant decrease in PDBu-stimulated release in neurons pre-treated with Bis I (p < 0.05, N = 10). Significance was determined using a two-way ANOVA with Tukey’s post-hoc test. N.S. – not significant.